Telomeres must overcome specific challenges to ensure their efficient replication. In yeast cells, telomeres are replicated in late S phase in agreement with the late firing of subtelomeric origins.

Figure 3: Autoradiogram of a denaturing polyacrylamide gel showing multiple-nucleotide insertions on 18-mer–28-mer primer–template duplexes (Fig. 1b) containing the analogs Z or F at the sixth ...